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KMID : 0350519920450020747
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Journal of Catholic Medical College
1992 Volume.45 No. 2 p.747 ~ p.759
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Thermosensitivity and thermotolerance of Acute Promyelocytic Leukaemic Cells (HL-60)
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Abstract
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Thermosensitivity in selective eradication of cancerous cells and thermotolerance during repeated heating are critical factors in the heat therapy of cancer. There has been no reports about heat shock protein synthesis in measuring
thermosensitivity of
single heat therapy and the change of thermotolerance depending upon themodes of double heat exposure.
So we studied the thermosensitivity by measuring the survivign fraction of human acute promyelocytic leukaemic cells (HL-60) and heat shock protein to investigate the correlation of heat shock protein synthesis and thermosensitivity.
Thermotolerance was
monitored by appearance of the heat shock protein and 3 modes of double heat exposure such as step-up(second heating at 43℃ after prior heating at 41℃ after at 45℃) were compared to investigate the most effective method for fecreasing the
thermotolerance.
@ES The results were as follows:
@EN 1. The surviving fraction of HL-60 was the least when it was heated at 43% C for 30 minutes.
2. Heat shock protein could be used as an index in measuring the thermosensitivity of single heat exposure and the main molecular weight of the heat shock proteins was 95KDa, 72KDa, and 70KDa respectively. Among those proteins 70KDa heat shock
protein
was the most sensitive to heating.
3. The thermotolerance of HL-60 was not induced when double heat exposure was siven by the mode of step down heating.
4. The thermotolerance induced by double heat exposure was lost after 24 hours at minimum.
5. The mainmolecular weight of heat shock proteins induced by double heat exposure was also 95KDa, 72KDa and 70KDa respectively. Among them 70KDa heat shock protein was the most sensitive one to idicate the development of thermotolerance.
With these results, it was considered to be significant to measure the thermosensitivity by surviving fractions of cancerous cells and heat shock proteins. Furthermore, it could be adapted in clinical trial that the mode of step-down heating is
the
most
effective to decrease thermotolerance.
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KEYWORD
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